Project

Part:BBa_K329019:Design

Designed by: Raphael Pantier   Group: iGEM10_Paris_Liliane_Bettencourt   (2010-10-24)


Population counter Tetracycline resistant - Final PmS-MTet


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1294
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3005
    Illegal BamHI site found at 1440
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1466
    Illegal NgoMIV site found at 1834
    Illegal NgoMIV site found at 1994
    Illegal AgeI site found at 3592
    Illegal AgeI site found at 3704
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3886
    Illegal BsaI.rc site found at 4614


Design Notes

We used BioBrick Standard Assembly.


Source

2010 Distribution kit and Mazel Lab.

References